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The manufacturer's intra-assay coefficient of variation (CV) based on samples assayed 12 times was ≤12.3%, and interassay CV based on samples assayed in duplicate in 11 different series was <14.2%.
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When adopting assays developed elsewhere, evaluation based on samples collected from the local population is necessary before the assays are used clinically.
Our main analyses are based on sample pairs with successfully assayed genotypes from both tissue sources.
The Z' values, a dimensionless measurement of assay quality based on sample means and their standard deviations, were calculated [24], [25].
The reproducibility rate of duplicated AM20 samples based on successfully assayed SNPs was 100% after excluding missing data.
Based on the sample assay results, three major active components among the six flavonoids, namely dracorhodin, (2S -5-methoxyflavan-7-ol and (2S -5-methoxyflavan-7-ollavan-7-ol, are suggested as the index for quality evaluation of DB medicine.
For HbA1c and selected blood lipids, assays based on DBS samples are clearly associated with assays based on standard venous samples.
Figure 1 shows antibody titers by time between onset and sample collection, based on HI assays for all 267 samples.
Based on these repeats, assay samples were subsequently indicated as positive when all three samples tested positive on a single assay, and negative when all samples were negative on a single assay.
based on a standard curve generated for each set of samples assayed.
The standard curve was based on triplicate assays, and extracted samples were each added to three wells containing cells.
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