Based on reversion rate measurements, we also found that the mutation rate increased linearly with increasing EMS concentration (Figure 1; R2 = 0.26, F1,196 = 68.62, P<0.001, the R2 value is low due to the substantial number of populations that did not exhibit reversion).
In order to examine if AID is able to trigger mutations in the absence of DNA replication in a non-B cell line, we developed a highly sensitive assay based on the reversion of nonsense mutations of the EGFP gene cloned in a non replicating vector.
Our system is based on the reversion of a nonsense mutation in an EGFP gene, cloned downstream of the CMV promoter/enhancer.
In the current study, we sought to identify epigenetically silenced genes in MCL using an initial genome wide screening based on pharmacological reversion of CpG methylation and gene expression microarray analysis in MCL cell lines followed by the analysis of selected methylated genes in primary MCL and normal B lymphocytes.
In order to obtain a more applicable approach to focus CTSSAR data, a novel imaging algorithm for CTSSAR is proposed based on the method of series reversion (MSR).
The Escherichia coli lacZ reversion assay, based on the set of episomal lacZ alleles engineered by Miller et al., provides an attractive system for studies of mutagenesis and mutational specificity.
The lys2-10A reversion assay is based on the restoration of the open-reading frame in a mononucleotide run of 10 adenines within the lys2 allele of strain RDKY3590 (Table 1), allowing mutant cells to grow on plates lacking lysine.
Revertants were initially identified based on eye color and reversion of the lethal insertion phenotype, then confirmed by PCR.
The model incorporated actual hospital costs (e.g., $8,023 for cesarean and $5,581 for vaginal delivery), utilities to quantify health-related quality of life, and probabilities based on analysis of published literature of successful ECV trial, spontaneous reversion, mode of delivery, and need for unanticipated emergency cesarean delivery.
