Sentence examples for based on reverse transcriptase from inspiring English sources
Based on reverse transcriptase domain search, 46 transposable elements (retrotransposons/retroposons) were identified (data not shown).
Both are positioned immediately downstream of ipbA and its σ32 heat shock promoter, and their response reflects transcriptional read-through based on reverse transcriptase quantitative PCR assays of different regions of the transcripts [ 22].
The APTIMA (GenProbe) and OncoTect (IncellDX) assays are based on reverse transcriptase (RT) and PCR technique and detect E6/E7 mRNA from 14 and 13 high risk HPV genotypes, respectively.
To determine ookinete infection in the infected tissue, we additionally performed a qualitative assay based on reverse transcriptase PCR (RT-PCR) for P. yoelii ookinete specific genes, pyCTRP [ 79] and pyECP1 [ 80] (for details refer Additional file 2).
Current diagnostics and IAV genotyping in pigs are based on widely used serological methods, such as the hemagglutinin inhibition assay (HI), neuraminidase inhibition assay (NI), and a broad range of molecular methods based on reverse transcriptase polymerase chain reaction (RT-PCR), multiplex RT-PCR (MRT-PCR), and real time RT-PCR (RRT-PCR) [ 23– 28].
VLP quantification and normalisation of concentration was based on reverse-transcriptase (RT) activity (Roche Colorimetric Reverse Transcriptase Assay, article 11468120910) which was put into relation to p24 content and international units based on the World Health Organization p24 reference standard (90/636).
In addition, phylogenetic comparisons among the reverse-transcribing viruses are usually based on their reverse transcriptase (RT) sequences and the lack of an RT sequence from ICE made such a comparison impossible.
The TRUGENE HIV-1 genotyping kit (Siemens, Tarrytown, NY) determined the HIV-1 subtype based on the reverse transcriptase sequence.
However, many of the studies were based on quantitative reverse transcriptase real time polymerase chain reaction (qRT-PCR) for selected genes with an a priori hypothesis.
All sequences from two previous large-scale analyses of plant LTR-RTEs [ 17, 24] were used to develop phylogenetic trees based on a reverse transcriptase fragment.
It is based on a reverse transcriptase polymerase chain reaction (RT-PCR) determination of the mRNA expression of 16 tumor-specific genes and 5 control genes.
