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Fig. 4 Quantification of intact Ga-FBP18 (a) and In-FBP19 (b) based on reverse phase HPLC-ICP-MS from single compound injection (filled triangles) and co-injection (open triangles).
A method based on reverse phase capillary high performance liquid chromatography (capillary HPLC) coupled to laser-induced fluorescence detection (LIF) has been proposed for the determination of ochratoxin A (OTA) in wine samples.
Here, a kinetic network analysis based on reverse phase protein arrays (RPPAs) in combination with extensive western blot and cell culture analyses was employed to decipher the cytoplasmic and nuclear signaling networks and to identify possible antiapoptotic pathways.
A comparison of retention times and fragmentation patterns, based on reverse phase chromatography for salicylic acid and HILIC for citramalic acid (see Methods section), proved that the root exudate signal with an m/z value of 137 [M-H]- originated from salicylic acid and that the signal with an m/z value of 147 [M-H]- originated from citramalic acid.
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Phospholipase activity was measured with a highly sensitive system that was based on reverse-phase high-performance liquid chromatographic separation of 9-anthracenylmethyl derivatives of fatty acids released by phospholipase A2.
The present study has indicated accurate and efficient analytical methods based on reversed-phase HPLC and spectrofluorimetry for the determination of SIL in a pharmaceutical sample.
Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction.
The enzyme was ≥97% pure based on reverse-phase HPLC. Figure 1A shows SDS-PAGE of the purified enzyme, with a major species of ∼55 kDa, and minor ∼40 kDa and ∼19 kDa species under reducing conditions, as described previously [23].
We optimized the FMNPs' reaction condition based on reverse micro-emulsion method through the Ternary phase diagram [48 50], finally obtained hydrophilic silica-coated CdTe QDs and MNPs with high fluorescent signal and magnetic intensity.
Hepcell is based on reverse passive haemagglutination technology.
The various forms of vitamin B12 in cytosolic and mitochondria enriched fractions were analysed by HPLC as described [8]. the method was adapted from Miller et al. [34] as described [35] and is based on a reverse phase high performance liquid chromatography technique using a linear acetonitrile gradient.
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