Sentence examples for based on restriction ligation from inspiring English sources

As a first step of our do-it-yourself protocol, we assembled monomers into multimers, using a procedure based on restriction, ligation and amplification.

Here we modified OE-PCR, referred to simultaneous splicing overlap extension PCR (SSOE-PCR), which was allowed to simultaneously assemble seven DNA fragments in one PCR reaction without the use of ligation based on restriction enzymes.

These methods are usually based on restriction digestion: genomic DNA is digested using one or several restriction enzymes, with simultaneous ligation of appropriate adaptors to the restriction fragments and subsequent amplification of fragments by PCR using the adapter and the restriction site as targets for primer annealing [ 6].

It is based on restriction fingerprints of 13,614 BAC clones.

Based on restriction fragment length polymorphism of the mitochondrial genome (employing eleven restriction enzymes), a polytomy was found [ 5].

2.Endoscopic therapy of bleeding esophageal varices is based on band ligation.

This technique is based on the ligation followed by PCR amplification of two adjacently hybridising oligonucleotides.

Briefly, the method is based on the ligation of oligonucleotide adaptors.

SNPlex is based on DNA ligation; therefore, its specificity is based on the characteristics of DNA sequence.

The HiC technique is based on proximity ligation and provides unbiased genome-wide maps of chromatin-chromatin association [ 106].

GoldenGate cloning is based on ligation of multiple inserts in sequential order through the action of type II restriction enzyme BsaI and of T4 DNA ligase in a single restriction-ligation reaction, followed by transformation into competent E. coli cells.