Sentence examples for based on restriction enzyme from inspiring English sources
All clones fell into one of two classes based on restriction enzyme patterns.
MSG reduces the genome complexity similar to other methods that are based on restriction enzyme digestion.
The two plasmids with different inserts were selected based on restriction enzyme (EcoRI or HpaII) analysis and used for sequencing.
Morales and Melnick [16], based on restriction enzyme analysis of a 1.6 kb mitochondrial ribosomal gene segment, found 0.3% sequence divergence between the white rhinos, and 1.8 2.1% between white and black rhinos.
We first compared DMH and MMASS, both of which are based on restriction enzyme digestion.
The sequence of pRB373 is unpublished but based on restriction enzyme digestion it contains a BsrG1 site.
Here we modified OE-PCR, referred to simultaneous splicing overlap extension PCR (SSOE-PCR), which was allowed to simultaneously assemble seven DNA fragments in one PCR reaction without the use of ligation based on restriction enzymes.
Despite the large diversity of existing DNA shuffling protocols, standard cloning methods based on restriction enzymes are not widely used in these protocols.
Since detection based on restriction enzymes is limited to fragments bearing the recognition sites [ 11, 48], adequate genomic coverage by MSRE requires a combination of nucleases [ 27, 44].
Moreover, studies focused on ancient mtDNA (González-Oliver et al. 2001) have shown the presence of haplogroup A2, B2, and C1 (following the most recent nomenclature) mtDNA lineages in pre-Columbians; and analyses of extant mtDNA based on restriction enzymes have also shown the presence of the four major Amerindian haplogroups in Mexican samples (Penaloza-Espinosa et al. 2007).
To investigate the dynamics of Vgt1 DNA methylation levels, we performed a preliminary analysis based on restriction with the methylation-dependent enzyme McrBC followed by qPCR, which showed a constant in time methylation peak in the central region of Vgt1.
