Sentence examples for based on restriction digestion from inspiring English sources

These methods are usually based on restriction digestion: genomic DNA is digested using one or several restriction enzymes, with simultaneous ligation of appropriate adaptors to the restriction fragments and subsequent amplification of fragments by PCR using the adapter and the restriction site as targets for primer annealing [ 6].

Their structures were verified based on restriction digestion patterns and Sanger sequencing.

In case of 5 nt microhomology substrates, only few junctions utilized microhomology (based on restriction digestion analysis), whereas majority of the joining was through C-NHEJ.

The dopamine D2 receptor (DRD2) was characterized previously with TaqIA, TaqIB, and TaqID SNPs based on restriction digestion of this region with the TaqI enzyme.

The size of the genome (281,132 nt) is similar to a previous estimation (255,000 nt) made by Robinson and Wolyn [ 43] based on restriction digestion mapping.

The number of D4Z4 repeats was inferred based on restriction digestion with EcoRI and XhoI enzymes and by q-PCR normalized to a single copy region, as described above.

MSG reduces the genome complexity similar to other methods that are based on restriction enzyme digestion.

We first compared DMH and MMASS, both of which are based on restriction enzyme digestion.

The sequence of pRB373 is unpublished but based on restriction enzyme digestion it contains a BsrG1 site.

Compared with other methods, RRL based on restriction enzyme digestion is relatively feasible and flexible, especially for those species without the reference genome [ 10].

However, a previous study by Shih and co-workers [ 56] outlined the existence of genetic variation in MCF viruses from different hosts based on restriction enzyme digestion patterns.