Sentence examples for based on restriction analysis from inspiring English sources

Selection of clones containing V136 or A136 was based on restriction analysis using BspHI (New England Biolabs Inc., MA, USA).

Based on restriction analysis with several four base cutters, the pENTR dTOPO clones derived from each species could be classified into 4 classes whereas the pBINPLUS clones displayed a single digestion pattern.

Plasmid DNAs were extracted and analyzed to differentiate the pTLU-type (telomere-associated linear) from the pRLU1-type (circular), based on restriction analysis and Southern blotting with a telomere probe.

Morales and Melnick [16], based on restriction enzyme analysis of a 1.6 kb mitochondrial ribosomal gene segment, found 0.3% sequence divergence between the white rhinos, and 1.8 2.1% between white and black rhinos.

First attempts of molecular typing of M. tuberculosis were based on restriction enzyme analysis of bacterial DNA (REA).

In case of 5 nt microhomology substrates, only few junctions utilized microhomology (based on restriction digestion analysis), whereas majority of the joining was through C-NHEJ.

There are 51 known HAdV serotypes classified into 6 species (A-F), based on restriction enzyme analysis and hemaglutination assays, later confirmed by genome analyses and phylogenetic calculations.

The first PCR based characterisation method based on restriction enzyme analysis of insertion sequence 1311 ISS 1311 PCR-REA) and Heat shock protein 65 gene, used limited amounts of DNA but could not distinguish between most isolates [ 8, 9].

Alternative methods have therefore been developed to detect these common mutations based on restriction enzyme analysis, allele specific amplification, digital PCR, fluorogenic hybridization probes, and high resolution melting (HRM) [ 6– 9].

We also found a few regions in the QTC4 DLD sequence that were different in the DLD transcripts from resistant insects that may be useful in designing a diagnostic assay based on restriction digest analysis for resistance in field populations of T. castaneum (shaded sequences, Additional file 6).

With respect to the pan-American haplogroups, we noted that the composition of Native Mexicans contrasts with the pattern reported by Salzano (2002) based on restriction endonuclease analysis, in which Mexican and Central American samples have higher frequencies of haplogroups A2 and B2 when compared with C1 and D1.