So far, only one linkage map based on restriction fragment length polymorphism (RFLP) markers was constructed using populations derived from heterozygous parental lines in yellow mustard [ 17].
All clones fell into one of two classes based on restriction enzyme patterns.
Here we modified OE-PCR, referred to simultaneous splicing overlap extension PCR (SSOE-PCR), which was allowed to simultaneously assemble seven DNA fragments in one PCR reaction without the use of ligation based on restriction enzymes.
Cleaved amplified polymorphic sequences (CAPS) markers are designed based on restriction fragment length polymorphisms of PCR amplified fragments, when sequence information of one of the haplotypes is unknown [ 6].
In Jeffreys's original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes.
MSG reduces the genome complexity similar to other methods that are based on restriction enzyme digestion.
Transformants were selected based on restriction site patterns and verified by DNA sequencing (Macrogen).
First, we describe an amplified fragment length polymorphism (AFLP complementary DNA (cDNA) display method that allows the discrimination of homoeologous loci based on restriction polymorphisms between the progenitors.
To provide more available molecular markers for this crop, a bioinformatic approach was employed to develop insertion deletions (InDels) markers in bottle gourd based on restriction site-associated DNA sequencing (RAD-Seq) data.
The method has been successfully validated in the context of complex in vivo and in vitro homologous recombination workflows, but it could be readily adapted to other cloning strategies, including those based on restriction endonucleases.
However, this contig was ∼7000 bp smaller than expected based on restriction digests.
