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The aim, based on replicating a rare naturally occurring mutation that makes some people resistant to HIV infection, is to turn off the CCR5 gene in patients' T-cells – a type of white blood cell important in the immune system.
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The predictive accuracies mentioned in the previous section are all based on replicated risk variants discovered by GWAS to date.
Brodsky et al[251] presented a method for evaluating individual peaks in a LC-MS spectrum, based on replicate samples.
For each gene at each time point we added values sampled from a Gaussian distribution centered on zero with a standard deviation calculated based on replicate measurements.
We also ran a two-way ANOVA test based on replicates.
Then data were filtered using the control strength, a control value calculated using the Cross-Gene Error Model based on replicates.
Systematic and random errors were evaluated based on replicate analyses.
Error bars for humic acid estimate uncertainty based on replicate analyses.
and standard deviations based on replicate experiments are used to determine which circuit best models the electrochemical data.
For experiments involving humic acid, sampling and filtration uncertainty is larger (~15%), based on replicate sample determinations.
Based on replicate analyses of laboratory standard, the analytical reproducibility is estimated to be better than ± 0.2‰ for carbon and better than ± 0.5‰ for oxygen isotope ratios.
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