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It illustrates a high patchiness, definitely supporting the need for more studies at vents based on replicated sampling designs.
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For CNV calling, we used PennCNV [ 9] as, for our data set we obtained a better reliability (0.65) based on replicated samples for this algorithm in comparison to other two algorithms [ 16].
For experiments involving humic acid, sampling and filtration uncertainty is larger (~15%), based on replicate sample determinations.
Brodsky et al[251] presented a method for evaluating individual peaks in a LC-MS spectrum, based on replicate samples.
Stable expression of reference genes was verified based on replicate samples (n = 4 from each group) with equal amounts of total RNA in each reaction analyzed using the 2-ΔCt method, and expression values normalized to the average Ct of three stable reference genes (CoxI, CyCTI-3, and Eif5a) using the ddCT method [ 52].
CIs were computed as bootstrap 95% percentile intervals based on 10000 replicated samples.
Experimental data fitted this model very well and Monte Carlo simulations based on this model replicated sampling experiments in a remarkable precision.
The analytical precision is ±0.1‰, based on replicate analyses of samples and standards (NBS-19).
The T-RFLP data were highly reproducible based on replicate extractions from the fecal samples and restriction digestions with three different restriction enzymes (data not shown).
For each gene at each time point we added values sampled from a Gaussian distribution centered on zero with a standard deviation calculated based on replicate measurements.
Systematic and random errors were evaluated based on replicate analyses.
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