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Additionally, miRNAs detected by microarray are based on reference sequences, however, the reference sequences are often not the dominant ones for some miRNAs, which might be another reason causing that discrepancy.
In some of the cases investigated here libraries based on reference sequences would outperform traditional tiling libraries.
Primers were designed based on reference sequences obtained from the Maize Assembled Genomic Island (MAGI) Database [45].
Those targets in Figure 2b) that do not indicate any degree of saturation with regard to 〈R〉 or do indicate saturation but not at an acceptable level of
(Based on reference sequences AY323264 and AY279022, for CCoAOMT1 and CCoAOMT2, respectively).
We constructed single map consisting of several types of markers (SSRs, DArT, SNPs and InDels) based on reference sequences of SSR, DArT and SNP markers.
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However, qRT-PCR widely used for miRNA quantification is mainly based on reference sequence [19].
cDNA numbering is based on reference sequence: GenBank NM_000038.
The data based on reference sequence similarity is taken from HomoloGene and cross-referenced.
Site numbers denote bp alignment positions of individual SNPs and starting positions of Indels based on reference sequence AY323283 of Genbank.
IGFBP7 primers (Additional file 1) were designed using Primer3 software [ 51] based on reference sequence (RefSeq) NM_001553 (IGFBP7), and on the genomic structure of the IGFBP7, as made available by the Human Genome Browser database [ 52].
More suggestions(17)
based on hemagglutinin sequences
based on reference architectures
based on promoter sequences
based on reference tags
based on cDNA sequences
based on reference values
based on reference conditions
based on reference signals
based on intron sequences
based on reference standards
based on video sequences
based on reference photographs
based on reference grammars
based on training sequences
based on globin sequences
based on reference datasets
based on reference models
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