Sentence examples for based on reference gene from inspiring English sources
Gapdh (glyceraldehyde-3-phosphate dehydrogenase gene) was chosen to standardize the quantitative experiments based on reference gene suitability testing.
The expression levels of each gene spot measured by the WEnCA method were quantified and then normalized based on reference gene (β-actin) density.
Sample cycle threshold (ct) values were standardized for each template based on reference gene control primer reaction, and the 2 ∆∆CT method was used to analyze relative changes in gene expression [ 28].
Although we have previously shown that qPCR may not necessarily be an optimal validation method for array data because it lacks endogenous reference controls and it is based on reference genes [ 19], it still provides an approximate corroboration of the information obtained with one platform.
Results were analysed using the qBase + data analysis software and expressed as normalized relative quantity (NRQ) [ 28] based on one reference gene for each tissue.
The search methodology mirrors that of CMAP in that the database consists of ranked lists of genes and it is interrogated with up and down regulated gene sets and query signatures are scored by a KS statistic with the significance based on reference to random gene set scores.
Based on references between genes in OMIM, we created connections between genes in QTLs identified in two distinct rat crosses.
ISGAP performed reliable annotation, recognizing accurate gene structures based on reference proteins, and ab initio gene models of the assembled transcripts.
The protein-coding genes in the transcriptome need to be extracted by the consideration of the gene structure, and ISGAP successfully performed annotation based on reference proteins and ab initio gene models.
We also introduced a novel metric to evaluate the functional significance of gene modules based on reference networks.
