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As a compromise, strategies based on reduced representation of the genome are currently more practical for whole-methylome analysis.
We developed an improved pipeline based on reduced representation bisulfite sequencing (RRBS) for cost-effective genome-wide quantification of DNA methylation at single base resolution.
RRBS is the first choice for DNA methylation analysis of clinical samples because of its minimal DNA requirements and low cost, based on reduced representation of the genome.
Recently developed Specific Length Amplified Fragment Sequencing (SLAF-seq) is an efficient method of large-scale genotyping, which is based on reduced representation library (RRL) [ 21] and high-throughput sequencing [ 22].
Previous efforts identified 438 target genes [ 77] from the annotated proteins set using the MEME and MAST algorithms [ 78] as well as 755 target genes for the NB-LRR gene repertoire [ 79] based on reduced representation analysis of DNA enriched (referred to as "Renseq" hereafter [ 80– 80]).
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The rest of the sum is based on reduced transfer fees, season ticket sales, merchandising and "lost business opportunities".
"Oh, so I've been selected based on reduced job status and perceived indolence?" "Yeah, something like that".
The association peak is located at an intergenic locus, which is flanked by two annotated genes [ Sb03g045420 (chromosome Sb03: 72,681,274 bp-72,687,688 bp) (similar to Hexokinase-3) and Sb03g045430 (chromosome Sb03: 72,703,668 bp-72,704,913 bp) (similar to Putative uncharacterized protein)], based on published reduced representation sequence [ 4].
Here, we used PEM on reduced representation libraries (RRLs) of pooled chicken DNA samples.
In what follows, we focus on reduced representation methods of genomic sampling such as RADseq and GBS.
Kerstens et al. (2011) performed paired-end sequencing on reduced representation libraries from pooled samples for four commercial chicken lines.
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based on reduced spending
based on reduced consumption
based on reduced operating
based on reduced heart
based on such representation
based on reduced dimension
based on reduced binding
based on reduced drug
based on reduced fertiliser
based on reduced sodium
based on spectral representation
based on reduced absenteeism
based on reduced information
based on reduced canine
based on proportional representation
based on reduced job
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