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Then, a genetic linkage group was constructed based on recombination frequency and SNP ordering was done using the ordering algorithm of RECORD (without imposing marker order) coming from REcombination Counting and ORDering, proposed by van Os et al. (2005a, 2005b).

In consequence there is only modest correlation between genetic linkage (based on recombination frequency) and physical distance, especially within large genomes (e.g. [13]).

Using each bin as a marker, a genetic linkage map based on recombination frequency was constructed, which was 1,625.5 cM in length, approximately 1.0 cM per bin, corresponding to 230 kb (Table S2), representing a great increase in marker density compared to 8.7 cM between adjacent markers in the RFLP/SSR map [16] and 2.4 cM/bin in the SFP map [9].

The lack of correspondence of some marker orders in our genetic map with the physical order in the sequence of cassava may have resulted from inaccurate estimations of genetic distance based on recombination frequency.

Early experiments unequivocally demonstrated the chromosome theory of heredity, provided important observations regarding the role of chromosome sites (including the centromere and the teleomere), and constructed the first meiotic map based on recombination frequency (reviewed in Lake and Hawley 2012).

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A genetic linkage map is constructed by placing genetic loci on chromosomes based on recombination frequencies, while the physical map is constructed based on overlapping restriction patterns of large insert BAC clones.

A genetic linkage map is constructed by placing genetic loci on chromosomes based on recombination frequencies, while a physical map is constructed based on overlapping restriction patterns of large insert BAC clones.

While in genetic maps, markers, genes or loci are ordered based on recombination frequencies at meiosis [ 38], physical maps present ordered fragments of cloned genomic DNA fragments and whose sizes and distances are given in base pairs (bp).

For the markers within each group, map calculations were performed in Joinmap based on recombination frequencies, which is the step when linked markers are placed on linkage groups and ordered relative to each other.

The first linkage map was calculated based on recombination frequencies of an experimental F1 × Pachón cave backcross pedigree using markers generated from random amplified polymorphic DNA (RAPD) fingerprinting (Borowsky and Wilkens 2002).

Further divisions could be based on recombination hotspots or just merely based on local proximity.

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