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JAFFA also provides a simple and effective method of ranking fusions based on read support and exon-exon boundary alignment.
However, it may be possible to filter the genome-guided Trinity results based on read support or expression level to yield a more manageable P. falciparum transcriptome assembly [Table 1, Additional file 9].
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The method is based on read and verify.
Read coverage was computed and genome annotation was challenged based on read distribution and coverage.
Nobody makes investment decisions based on reading financial filings.
Discordant scores based on reading errors were corrected.
Plasmids were putatively identified based on read-pairs supporting circularization, gene content (e.g. plasmid replication genes) and read coverage.
Few methods to study AS based on read assembly exist.
Next, p values were calculated using Fisher's exact test for all putative mutation sites, based on the distribution of read support with high stringent specificity and sensitivity [ 11].
Based on the sequencing reads supporting inclusion and exclusion events, MISO (mixture of isoforms) [ 17] is designed to estimate the percentage of inclusion for every previously documented alternative-splicing event in a sample.
Cufflinks [8], its sister tool, assembles mapped reads into a parsimonious set of transcripts and then estimates the relative abundances of these transcripts based on their supporting reads.
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