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Sequences were assembled using the Phusion assembler [ 18], based on read pair information.
Genomic DNA from Pa ATCC43949 was sequenced by a shotgun approach and sequences were assembled using the Phusion assembler [ 18], based on read pair information.
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The resultant contigs were then joined into scaffolds based on read-pair associations and order information from the shotgun clones.
Plasmids were putatively identified based on read-pairs supporting circularization, gene content (e.g. plasmid replication genes) and read coverage.
Scaffolds were formed using the Bambus software [ 98] based on read mate-pairing.
Chromosomal rearrangements were investigated through 4 independent analyses based on read depth, copy number variation, pair-end reads and PCR.
"Butterfly" processed the individual graphs in parallel, tracing the paths based on reads and pair-end information, ultimately reporting full-length transcripts for alternatively spliced isoforms.
SVs were predicted based on the read pair information from the paired-end sequenced small insert RRL, which was purposely created for SNP detection.
Next, we applied F-seq, a feature density estimator [ 34, 35], to compute PA cluster based on uniquely mapped read pairs.
To compensate for this bias we required that SVs, predicted based on discordantly mapping read pairs that mapped to the reference between 220 and 720 bp apart, meet a representation constraint.
Maximum variety sampling (Lincoln and Guba 1985 ) of evaluations based on reading and summaries of each report by pairs of reviewers.
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