Sentence examples for based on read mapping from inspiring English sources
Contigs from composited metagenomes were binned into metagenome-assembled genomes (MAGs) using MetaBAT (; v. 0.18.6) based on read mapping with Bowtie2.
GMS is a statistical measure for the probability that a position is at risk of being multialigned, based on read mapping.
A total of 77 samples were initially processed, of which, only 70 passed quality control based on read mapping and base level quality scores.
Finally, we manually checked the sequences of all the candidate genes based on read mapping and visualization in Integrative Genomics Viewer (IGV, v2.3.40) [ 80].
With our deep sequencing data, we were able to follow the latest microRNA annotation guidelines [ 9] to integrate sequencing data with MIRNA annotation and exclude many false MIRNA loci which are more likely siRNA loci based on read mapping patterns.
While read mapping provided the initial clues about HEs, the precise locations and boundaries of HEs across a set of lines were difficult to assess based on read mapping, thereby requiring a tedious validation procedure based on PCR and targeted sequencing in the study reported [ 27].
Based on read map coverage, the relative abundance of lncCR-H was comparable to that of mitochondrial mRNAs (e.g. ND4L mRNA [ 29]).
Genome coverage based on k-mer exceeds 105%, and genome coverage based on reads mapping was 97% for the three genomes.
Peak calling was performed by MACS version 1.4 [ 26] using shift size determined by the size of sequencing library inserts, and switching off local background estimation (as recommended for histone modification peak calling in the absence of a control immunoprecipitation). Peak calling was only based on reads mapping to a single location, excluding duplicates.
Here we introduce a novel tool (HLAreporter) for HLA typing from NGS data based on read-mapping using a comprehensive reference panel containing all known HLA alleles, followed by de novo assembly of the gene-specific short reads.
Introducing additional filtering based on read-mapping statistics further improved specificity without reducing sensitivity, in particular with filters based on the fraction of reads that were mate-pair rescued.
