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In addition, one can generate a taxonomic profile by quantifying the assigned reads, based on read counts or coverage for each individual bin.
To eliminate the influence of different gene lengths and sequence discrepancies on expression calculations, gene expression levels based on read counts obtained by RSEM (version v1.2.15) were normalized using FPKM (fragments per kilo bases per million fragments) transformation (Li and Dewey 2011).
Furthermore, we have a basic algorithm to derive a set of coordinates from DS data based on read counts or sequencing coverage.
Second, one can also compare the average expression level among gene families or individuals (biological replicates) based on read counts.
Differentially expressed genes were selected using the Bioconductor package DEGSeq based on read counts following a Poisson distribution model [ 86].
Gene expression levels were calculated based on read counts which also included unambiguous mapped reads according to negative binomial distribution.
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The most abundant (based on read count) RNA species in both libraries were classified as miRNAs, representing 74.52% of delayed implantation library and 76.57% of activation library, respectively (Table 1).
Another type of RNAseq differential gene expression analysis is based on read count.
The current version of MultiRankSeq only considers methods based on read count data.
Although 454 technologies have low sequencing depth, we estimated the NBS-LRR gene expression profiles based on read count.
TSSs were identified by a significant increment in read count compared to average background and quantified based on read count over the subsequent 50 bp.
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