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Objectives: To compare different assays for CMV detection, especially assays based on (quantitative) DNA and mRNA detection.
Recent advances in our understanding of the complex regulation of FoxP3 expression have led to new methods of analysing Treg based on quantitative DNA methylation analysis of FoxP3 locus [ 13 ], which may add a useful test for quality assessment of ex vivo manipulated Treg cells.
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Our study was based on quantitative analysis of DNA methylation using pyrosequencing, which is highly reproducible and accurate at measuring small changes in DNA methylation (Bollati et al. 2007; Yang et al. 2004).
All the results in the present study were based on quantitative signals of the DNA methylation.
Estimates of family contributions to the pools based on quantitative genotypes in pooled DNA had a correlation of 0.85 with estimates of contributions from DNA-derived pedigree.
Endolysosomal escape of DNA was documented at different time points based on quantitative analysis of colocalization between fluorescently labeled DNA and endosomes and lysosomes.
Here, we identify this repressor which we call muscle growth regulator (MGR)—by using a DNA protein interaction screen based on quantitative mass spectrometry.
In conclusion, we demonstrate a binding model based on quantitative estimates of the binding affinity of TLR9 dimers with CpG and non CPG DNA sequences in live HEK 293 cells.
Furthermore, based on quantitative PCR (qPCR) analysis, animals with BU lesions (and some without) shed M. ulcerans DNA in their faeces, indicative of bacterial loads of up to 108 organisms/gram.
Through a strategy based on quantitative real time PCR (Boulay et al, 1999, 2001), we performed genetic analyses of corresponding DNAs by copy dosage of the SMAD4 gene.
holdings more conservatively — resulting in losses — much of their methodology was based on "quantitative evaluation".
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