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With this monitoring, a metric on overall process variability was assigned for the platform's performance based on quantitation of metabolites in actual experimental samples (see results section).
CellTiter-Glo is a homogeneous method of determining the number of viable cells in culture through generation of a luminescent signal based on quantitation of the ATP present, an indicator of metabolically active cells [ 15].
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We estimated that <1% of the segregants were h + cells, based on quantitations of mating frequency in the mutant pool.
Based on quantitation cycle values, automated high-throughput extraction of samples proved to be as suitable as manual extraction.
The atom balances were determined using all of the gaseous decomposition products based on quantitation by multivariate regression methods.
CellTiter-Glo kit determines the number of viable cells based on the quantitation of adenosine triphosphate, which signals the presence of metabolically active cells.
The CellTiter-Glo luminescent cell viability assay (Promega, Mannheim, Germany) is based on the quantitation of ATP, reflecting the presence of metabolically active or viable cells.
This scheme is based on accurate quantitation of sensitized emission in a set of calibration strains, and then using a calibration between sensitized emission and FRET efficiency to deduce the donor acceptor separation in experimental samples.
On the other hand, this finding confirms the necessity of developing a comprehensive quality evaluation standard based on the quantitation of the active components and on novel analysis technology to ensure the quality of DB medicine.
Gated live hemocytes are analyzed and sorted at will based on precise quantitation of fluorescence levels originating from metabolic indicators, lectins, reporters (GFP and beta-galactosidase) and antibodies.
These observations were confirmed in a second assay based on the quantitation of hydroxyproline in procollagen by reverse phase HPLC (Fig. 3k) and provided strong evidence that mTORC1 is a key signaling node involved in mediating the potent fibrogenic effects of TGF-β1.
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