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Based on quantitation cycle values, automated high-throughput extraction of samples proved to be as suitable as manual extraction.
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The atom balances were determined using all of the gaseous decomposition products based on quantitation by multivariate regression methods.
Based on quantitation, lesions were similarly classified as regressive (>30 % decrease), progressive (>30 % increase), or stable (rest).
With this monitoring, a metric on overall process variability was assigned for the platform's performance based on quantitation of metabolites in actual experimental samples (see results section).
We estimated that <1% of the segregants were h + cells, based on quantitations of mating frequency in the mutant pool.
According to the manufacturer's instructions (Applied Biosystems, PPARR γ mRNA quantitation was based on the comparative cycle threshold (Ct) method using ribosomal 18S RNA expression for normalisation.
The solution based on duty cycle.
For each run, dilutions of BB7 or JFH-1 plasmid DNA (precisely quantitated using the RiboGreen DNA quantitation kit (Invitrogen)) ranging from 0 107 copies per well were run in triplicate to generate a standard curve, which served as a reference to calculate HCV RNA copy number based on the cycle threshold (Ct).
Quantitation via real-time PCR is based on cycle threshold (CT).
Quantitation of the PCR results were calculated based on the threshold cycle (Ct) and were normalized using β-tubulin for all mouse transcripts or TATA Binding Protein TBPP) for all human transcripts.
Quantitation of the PCR results were calculated based on the threshold cycle (Ct) and were normalized using TATA Binding Protein as a control.
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