Sentence examples for based on pull down from inspiring English sources
The results showed protein M could be a superior affinity reagent for IgY, scFv as well as IgYΔFc, based on pull down and western blot investigations; in addition, it was found that ∼125 times increase of effective IgY in the elutent was obtained using protein M affinity chromatography column compared with traditional IgY extraction methods.
Since Munc18-1 is also able to bind in an alternative mode to the assembled SNARE complex [12], [13], [15], [32] we also tested the binding of Glu379 in an assay based on pull down of assembled SNARE complexes from a rat brain extract using GST-complexin.
Therefore an experimental approach based on pull-down of endogenous proteins was used in a reciprocal way.
This data was collected by a high throughput experimental approach based on pull-down technology applied to E. coli proteins.
One biochemical assay is based on pull-down with immobilized GST-Nck, and has served to demonstrate that certain stimulatory anti-CD3 antibodies [17] and a panel of pMHC ligands [33] induce a conformational change in the TCR.
Each Skp1 isoform was capable of high-affinity binding to a natively soluble F-box protein, Fbs1, based on pull-down studies, indicating that the modifications are not required for FBP interaction as previously shown for FbxA.
Based on pull-down assays, the C-terminal consensus PDZ-binding motif (ITEL) of cadherin-23 had previously been reported to be involved in the binding of cadherin-23 to harmonin, and the absence of the exon 68 encoded insert has been proposed to enhance this binding, possibly by unmasking an internal PDZ-binding sequence (17).
We applied the method to detect protein targets of small-molecules based on affinity pull-down experiments and to identify differentially regulated proteins after microRNA over-expression or repression.
Based on previous pull-down experiments, we had suggested that formation of the closed state of TAp63α involves both the N-terminal transactivation domain (TA) and the C-terminal TID (Serber et al., 2002).
However, unlike the aforementioned kinases that bind to YopJ, IKKα, which does not bind YopJ based on in vitro pull down assays, has a phenylalanine at this position [16].
