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The methods for protein extraction, followed by western blot analysis, were based on protocols from previous studies22,48.
hBMSCs were isolated and cultured based on protocols from previous studies [ 11, 12].
It is based on protocols from mammalian subcellular preparations (Huttner et al., 1983; Ahmed et al., 2013).
A prevalence study based on protocols from maintenance claw trimmings demonstrated that 72% of Swedish dairy cows had at least one claw lesion [ 3].
To assess the biological activity, of the compounds shortlisted by the in vitro screen on HIV-1 replication, we employed a viral replication assay based on protocols from Hu et al. (Table S2, Supporting Information).
Specimens for in situ hybridization were anaesthetized in tricaine methanesulfonate (MS222, Argent) and fixed overnight in 4% paraformaldehyde (PFA) in 0.1% phosphate-buffered saline (PBS) at 4°C. Whole-mount in situ hybridization experiments were based on protocols from [ 12] and modified as follows: embryos were transferred to methanol for dehydration and stored at -20°C.
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Chemotherapy is, therefore, chosen for an individual patient based on protocols developed from observations from prior clinical studies on other patients.
We investigated subgroup planning and reporting based on protocols of randomised controlled trials from six international centres and the corresponding publications.
The enzyme assays were conducted based on protocol adapted from the literature, considering several variables [ 12– 12].
Summative mass closure of the biomass was obtained following the protocol established by Rocha et al. [ 32], developed specifically for sugarcane bagasse, and based on standard protocols from the National Renewable Energy Laboratory [ 49, 50] and also on a validated methodology from [ 51, 52].
In Family 1, we isolated mRNA using the RNeasy kit (Qiagen, Hilden, Germany) from lymphoblastoid cell lines that have been established based on standard protocols from patients MR026-01 and MR026-04.
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