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This is a retrospective study based on protocols describing eye examinations made during the years 1997 - 2001.
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The set of procedures applied here is based on protocols described in IAEA (1991, 2003, 2010) and by Grasty and Minty (1995).
Total RNA isolation and cDNA synthesis were performed based on protocols described previously [ 20].
All methods were based on protocols described previously by Hardy and Townsend (Hardy and Townsend, 1994).
Isolation and verification of hUC-MSC were carried out based on previously described protocols, and determination on Ad- HGF optimal transfection efficiency was performed based on protocols described previously [ 14].
HER2Mab was constructed as a recombinant human IgG1 based on variable domains of the anti-HER2 antibody rhuMAB 2C4 (pertuzumab) [ 7] and expressed transiently in HEK293 cells and purified based on protocols described previously [ 10].
Samples for HOPE were amplified based on protocol described previously with forward 11F [5'-GTT YGA TYC TGG CTC AG-3'] and reverse 1492R [5'- GGY TAC CTT GTT ACG ACT T-3'] primers [25].
For GUS histochemical assay of transgenic Arabidopsis, leaves from T2 plants were soaked with staining solution containing 1 mg ml-1 of 5-bromo-4-chloro-3-indoyl-β-d-glucuronide (X-Gluc) based on protocol described by [ 44].
Microvessel isolation based on protocol previously described by Brooks et al. [33].
BMSCs were obtained from femur bones and PTSCs were isolated from patellar tendons of seven New Zealand white rabbits (4 6 months old females) based on protocols similar to those described previously.
The PCR was conducted based on the protocols described by Liu et al. [ 9].
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