Sentence examples for based on protocol described from inspiring English sources

Samples for HOPE were amplified based on protocol described previously with forward 11F [5'-GTT YGA TYC TGG CTC AG-3'] and reverse 1492R [5'- GGY TAC CTT GTT ACG ACT T-3'] primers [25].

For GUS histochemical assay of transgenic Arabidopsis, leaves from T2 plants were soaked with staining solution containing 1 mg ml-1 of 5-bromo-4-chloro-3-indoyl-β-d-glucuronide (X-Gluc) based on protocol described by [ 44].

The set of procedures applied here is based on protocols described in IAEA (1991, 2003, 2010) and by Grasty and Minty (1995).

Total RNA isolation and cDNA synthesis were performed based on protocols described previously [ 20].

All methods were based on protocols described previously by Hardy and Townsend (Hardy and Townsend, 1994).

Isolation and verification of hUC-MSC were carried out based on previously described protocols, and determination on Ad- HGF optimal transfection efficiency was performed based on protocols described previously [ 14].

HER2Mab was constructed as a recombinant human IgG1 based on variable domains of the anti-HER2 antibody rhuMAB 2C4 (pertuzumab) [ 7] and expressed transiently in HEK293 cells and purified based on protocols described previously [ 10].

This is a retrospective study based on protocols describing eye examinations made during the years 1997 - 2001.

Microvessel isolation based on protocol previously described by Brooks et al. [33].

An optimised In situ hybridization method for the detection of FMDV was developed using digoxigenin-labelled RNA probes based on the protocol described by Prato Murphy et al [9] and optimised for frozen sections incorporating pre-hybridization blocking steps, tyramide signal amplification and alkaline phosphatase based visualization as described by Yang et al [40].

SP-PCR was done based on the protocol described in ref. 70.