However, to make a useful and robust multi-marker test based on protein profiles, test development and validation should include the use of animals that originate from different breeds, different farms, different time points post infection and from animals with different disease history.
After the rise of molecular genetics in the 1950s, the field of molecular evolution developed, based on protein sequences and immunological tests, and later incorporating RNA and DNA studies.
Thus, newer serological tests based on protein antigen and or combination of protein antigen by combination of suitable IgM, IgG, or IgA may eventually overcome such difficulties.
First, we tested our ability to distinguish the human and chimpanzee samples based on protein expression.
The aim of this study is the development of an ELISA test (kit) based on proteins smaller than 10 kDa (specially ESAT-6) for the detection of TB in cattle.
The main difference between the two modes of cross-validation is that the train-test split is based on proteins in the first mode and protein pairs in the second mode.
Once the blood plasma is "melted," it produces a unique signature based on proteins in blood.
The differences seen between these curves are comparable to the difference one would expect to see, based on similar tests of protein-protein searches[ 7].
As prediction of the sensitizing potential of the novel introduced protein based on animal testing was considered to be very important, animal models were introduced as one of the new test items, despite the fact that non of the currently studied models has been widely accepted and validated yet.
Protein candidates were selected into the signatures based on significance testing and a stepwise protein selection, each within 10-fold cross-validation.
