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(B – C ) Determination of stoichiometry based on protein standards.
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The theoretical molecular weight of multimeric glycoproteins is higher than that determined based on Protein Ladder standards, because the samples were resolved in reducing conditions.
The tissue concentration of BDNF was calculated and standardized based on protein concentrations of the spinal cord samples and recovery rate of the internal standard sample [73].
In yeast, one proposed source of gold standard negatives is based on protein localization data [ 23, 25] because pairs of proteins localizing to different cellular compartments are highly enriched for non-interacting proteins.
Internal standards were added based on protein concentration and included 16:1-16:1 PtdEtn (100 nmol/mg of protein), 14 1-14:1 PtdCho (45 nmol/mg of protein), T14 0 cardiolipin (3 nmol/mg of protein), 15:0-15:0 PtdGro (7.5 nmol/mg of protein), 14 0-14 0 PtdSer (20 nmol/mg of protein), 17:0 lysoPtdCho (1.5 nmol/mg protein), N12 0 CerPCho (20 nmol/mg of protein) and N17:0 Cer (5 nmol/mg of protein).
Caspase-3 activity was re-calculated from the slope (fluorescence units per time) and is given as picomoles of substrate cleaved per milligram of protein per minute based on a standard curve for 7-amino-4-methylcoumarin (AMC; Biomol, Germany).
Once the blood plasma is "melted," it produces a unique signature based on proteins in blood.
It has been previously suggested that it is challenging to derive high confidence identifications of human serum/plasma proteins [ 4] and that most proteomic identifications are false positive results using the "empirical model" based on putatively pure protein standards verses a decoy library [ 24].
RNAMotifModeler also provides an option to predict only sequence consensusmotif based on gold-standard protein-RNA binding sequences.
ELISA data were analyzed using absolute concentrations based on the recombinant protein standard and fold increase over the control wells on the same culture plate.
These restrictions are encoded in the ontology based on global standards (SI [ 21]), where protein properties are measured by specific units of measurements.
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