MALDI-TOF mass spectrometry (MS) has been suggested as a fast and reliable method for bacterial identification [10], [11], based on protein profiles characteristic of each microorganism.
Levels of individual proteins were not extremely elevated in our study subjects, but based on protein profiles subjects may be differentially classified with lower or higher risk to develop a more pathological phenotype.
A long-term goal is to predict clinical outcome, based on protein profiles present in the serum early in the disease course.
Alternatively, methods based on protein profiles account for the fact that sequence conservation might be very uneven across the length of two functionally related genes.
Categorization of samples based on protein profiles has the potential for downstream applications in a clinical setting for subclassification of glioma in patients, as was previously accomplished using miRNA expression levels.
Based on protein profiles we predicted that P. novae-zelandiae would accumulate more C4 glucosinolates than the other two species and would produce nitriles in hydrolysis instead of isothiocyanates.
It contains ∼97% of the strains included in the database and is more reliable than the 'MLST+AFLP+MLEE' supertree because MLEE, which is based on protein profiles, has a much lower resolution power than MLST and AFLP.
However, to make a useful and robust multi-marker test based on protein profiles, test development and validation should include the use of animals that originate from different breeds, different farms, different time points post infection and from animals with different disease history.
This property does not hold for scoring functions based on protein signature profiling, making this unattractive.
Based on protein expression profiles of core regulatory proteins involved in the G1-S and G2-M phase transitions, we have identified three distinct cell cycle phenotypes in a series of 200 breast cancers: a G0 out-of-cycle state (18% of cases); a G1 arrested/delayed state (24% cases); and accelerated S-G2-M phase progression (58% of cases).
