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Signatures based on protein levels are starting to be developed [6].
The aMØs' response to receptor ligation was quantified based on protein levels of cytokine-induced neutrophil chemoattractant-1 (CINC-1), macrophage inflammatory protein-2 (MIP-2), tumor necrosis factor- α (TNF- α), macrophage chemoattractant protein-1 (MCP-1), and interferon- β (IFN- β) in the culture supernatant, as assessed by ELISA.
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Therefore, regulation based on protein level does not explain its role in pseudohyphal differentiation.
For instance, the target gene of miRNA477 in P. patens has several different types of predicted functions (based on protein level sequence homology), including heat-shock protein related, MRP-domain ribosomal protein L29-like and Zinc-finger CCT-domain proteins.
InParanoid v4.1 [ 48] was then used to identify orthologous sequences between clades based on protein-level similarity.
Under this method, a mapping of GO terms and domains is constructed based on protein-level GOA and domain compositions of proteins.
Fang and Gough [ 41] generalized a dcGO predictor for inferring GO terms associated with individual domains and supradomains based on protein-level GO annotation (GOA) and families of protein.
Second, all markers were evaluated based on protein expression levels, not on genetic levels, which means the results do not reflect molecular changes in cancer cells on a genetic level.
The apparent Km, Vmax, and corrected Vmax values (calculated based on protein expression levels) have been summarized in Table 2.
Differences in DSS based on protein expression levels were plotted using Kaplan Meier survival curves and evaluated using log-rank tests.
This suggests that transcriptome alterations may not be fundamentally based on protein expression levels or insert complexity, but instead could be due to the attributes of the specific recombinant protein being expressed, mutations/disruptions from the insertion of T-DNA, or some combination of both.
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