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Maximum likelihood phylogenies based on protein data were computed with PHYML (Guindon et al. 2005) with 100 bootstrap replicates.
The best matches (e-value ≤ 10-4) were compared with the UCSC genome browser 'known gene' annotation (hg18), which is based on protein data from UniProt and mRNA data from the NCBI RefSeq collection and GenBank.
This table shows the comparison of the number of essential proteins identified by ION and seven other existing centrality methods (DC, BC, CC, SC, EC, IC and NC) based on protein data from E. coli.
The UCSC Known Genes dataset is based on protein data from Swiss-Prot/TrEMBL (UniProt) and the associated mRNA data from GenBank, and serves as a foundation for the UCSC Genome Browser.
Phylogenetic analysis based on protein data of 12 OXPHOS genes resulted in a robust phylogeny with the exception of the Dothideomycetes and Leotiomycetes which clustered together, but their position was not resolved and it remained unclear whether they share the most common ancestor with the Sordariomycetes or the Eurotiomycetes.
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In yeast, one proposed source of gold standard negatives is based on protein localization data [ 23, 25] because pairs of proteins localizing to different cellular compartments are highly enriched for non-interacting proteins.
The clustering of samples based on protein expression data did not lead to clusters separating benign from tumor specimen but rather identified a subpopulation of samples that formed a unique cluster.
The same association was found in a more recent paper, based on protein profiling data [ 25].
4.Thg1p of budding yeast seems to form a homodimer based on protein interaction data from two independent experiments.
We also examined a subset of the negatives from those screens that we identified as possible false negatives based on protein interaction data.
Pathway annotations of organisms other than human are derived by mapping their human counterparts onto these organisms based on protein orthology data.
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