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If one studies phylogenetic relationships based on protein coding genes in bacteria, it is possible to encounter the outcome of horizontal gene transfer (HGT) [ 40].
Based on protein coding genes (pseudogenes are not included) downloaded from 28 LAB and one B. subtilis genome sequences, a search for orthologs was conducted with the program Inparanoid version 2.0 [ 14].
Phylogenetic analysis based on protein coding sequences then aligned the microsporidia within the fungal kingdom [10], [11], [12], [13], [14], [15], and the lack of mitochondria was soon undermined by the discovery of a reduced "mitosome" in microsporidia [8], [16].
In A431 and U2OS, these splicing mutation predictions enhance and strengthen the pathway analysis based on protein coding mutations alone.
For the soybean genome, current gene models using the 8× genome sequence assembly (version Glyma1, [ 46]) were predicted based on protein coding sequences.
When the region is extended to around (-1500,+100) and the FP rate is fixed at 20%, the TP rates for human reached 80% for the permuted negative samples, 60% for the randomly generated intergenic negative sample, but only 44% for the negative samples generated based on protein coding DNA sequence.
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Moreover, the cluster of samples from the posterior based on tRNA-coding sites seem to be farther away from that based on protein-coding sites.
Representatives of all bacterial phyla were detected in the seven libraries based on protein-coding transcripts indicating that viable microbiota were present in lymph nodes.
An interesting corollary to this is the fact that splice signal sequences for tRNA and rRNA genes that are not based on protein-coding sequences do not include stop codons [32], [33] – further suggesting that the stop codons at the ends of coding exons may have arisen from the ORF ends in random DNA sequences.
Thus, the gene duplication events observed in this study could not be dated based on protein-coding sequences.
CR haplotypes were also generally congruent with phylogenetic reconstructions based on protein-coding regions within the sperm whale, albeit with less resolution in the CR haplotypes (fig. 1).
More suggestions(15)
based on protein metabolism
based on protein abundance
based on turbo coding
based on protein amount
based on network coding
based on superposition coding
based on protein content
based on protein sequence
based on waveform coding
based on protein level
based on protein staining
based on protein homology
based on subband coding
based on protein migration
based on protein domain
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