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A more detailed dissection of the patterns of gene gain and loss in the E. coli and Shigella based on progressiveMauve alignments has been given elsewhere [55].
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We then constructed multiple alignments of peptide sequences using ClustalW and constructed nucleotide alignments based on the peptide alignments.
We introduce a new set of assembly accuracy metrics based on the progressiveMauve genome aligner (Darling et al., 2010).
The SILVA seed alignments are based on alignments published by the ARB project in 2004.
Based on these alignments PCR primers were designed.
All the above comparisons were based on Bowtie2 alignments.
Alignments of nucleotide sequences were constructed by PAL2NAL [ 112] based on protein alignments.
Based on the alignments, a maximum likelihood tree was constructed.
Two families were identified based on LTR alignments.
Single nucleotide polymorphisms (SNPs) were detected based on these alignments.
DNA alignments were obtained using DNA sequences, based on protein alignments for the gene families.
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