Our developed screening based on probes for the major classes of AMR mediated by enzymes or efflux proteins extends previous studies [ 14] in complexity and analysis methodology.
MLPA is a mRNA quantification assay based on probes, each of which consists of two unique oligonucleotides of specific lengths, one unlabeled and one fluorescently labeled, that anneal to adjacent sites on a specific target sequence.
Furthermore, it avoided the decrease of affinity, which was encountered frequently in the conventional approaches based on probe labeling of McAb and protein conjugation of hapten.
The combined p and median t methods are both based on probe level tests of significance and perform well based on ranking genes in order of evidence of differential expression.
It should be clear that a probe set level normalization does not apply to methods based on probe level tests of significance.
This agreement is most likely due to the fact that these three testing methods are based on probe values instead of expression indices.
The combined p and median t methods are both based on probe level tests of significance and seem to rank genes similarly in many cases.
The comparative analysis detailed here was based on probe annotation and aspects of the relevant underlying biology.
Top probe sets were selected based on probe set specificity, strength of association to the Comt locus, biological function, and expression pattern for each relevant brain region in the AIBS (www.brain-map.org).
In[15], two stages for scheduling process based on partial probing of the users has been presented, in order to reduce the feedback, at the second stage the probing process stops and only the remaining users are requested to feedback their channel quality.
To reduce the likelihood of non-target hybridization, we designed a workflow for finding the best probes of input sequences based on uniqueness of probe sequence and probe-to-target duplex stability.
