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Two differentially expressed transcripts from two genes were selected based on probe set size consideration.
Top probe sets were selected based on probe set specificity, strength of association to the Comt locus, biological function, and expression pattern for each relevant brain region in the AIBS (www.brain-map.org).
Recently, Möller-Levet et al. introduced the new metric VFC which is a weighted fold-change based on probe set reliability, i.e. detection above background score [ 33].
We acknowledge that, in all likelihood, testing methods based on probe set summary values would have exceeded the performance of the methods based on probe level tests had a probe set level normalization been performed for the Golden Spike data.
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Entities were filtered separately for each time point based on probe sets as "detected" or "not detected" in at least 1 of 3 biological replicates using Genespring GX 12.5.
In O2EM, significant differential expression is summarized according to gene annotation based on probe sets, genes, transcripts or protein levels as it is selected by the user on the tool's entry page.
In this study, unsupervised hierarchical clustering of FTE and HGSC based on probe sets corresponding to all (both ligand-dependent and -independent) PR-A-dependent genes resulted in two main cluster groups (I+II), each of which further divided into two subgroups (a+b; Fig. 5).
To homogenise them, the data were cross-mapped based on probe-sets and scaled on median expression.
Considering that an expression signal below a cut-off of 3.5 after GC-RMA normalization cannot be distinguished from noise or missing signal, the present analysis was based exclusively on probe sets with a signal level less than 3.5 in no more than 85% of the samples analyzed: 29,683 out of 54,675 probe sets were included in the analysis.
To determine whether this annotation could indeed be used to filter for probe sets detecting NAT expression, we developed an automatic classification algorithm based on the probe set mapping to the Ensembl v36 genome release [ 25].
In this study, predictions based on sufficient probe set size and fold-change are confirmed by independent means.
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