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Among a pool of four purchased siRNAs against ROCK2 and LIMK1 (Qiagen, Hilden, Germany), the most effective siRNAs were chosen based on prior testing in cell culture.
We chose a phosphate buffering system to set pH of the sample to 7.4 based on prior testing and in accordance with other published buffering methods [ 4, 31].
The value of N for a given shape, designated as the skip factor, was based on prior testing that established what spacing would provide equally recognizable shape patterns.
The mutation profiles of these samples were known based on prior testing using the Ion Torrent Personal Genome Machine (46-gene hotspot panel), Sanger sequencing, or fluorescence in situ hybridisation (FISH).
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Percentile levels are divided into categories with descriptive labels based on prior test findings from different populations, normal and impaired.
The generated reads are then treated with bioinformatics tools based on prior knowledge of tested GMO.
Based on prior experience, we tested log-transformed metabolite concentrations (Biocrates and Lipofit platforms) or ion counts (Metabolon platform) for association with DNA methylation (b-values), using age, gender, body mass index (BMI) and white blood cell count (WBC) as covariates in a linear model.
MRI parameters were determined based on prior experience and additional test scans including quantitative T1 and T2* maps.
('yes' or 'no'), The spinal pain questions were based on prior questions with demonstrated test-retest reliability and validity in comparison to clinical interview[ 8, 35, 36].
Worse-value treatments are as good and more expensive, or worse and the equally or more expensive ** Validating studies test the quality of a specific diagnostic test, based on prior evidence.
Additionally, tests based on prior data from the literature (e.g., the interaction between SLC19A1 genotypeotype and MTHFR C677T genotype) were not corrected for multiple testing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com