Sentence examples for based on primers published from inspiring English sources
Primer sequences are based on primers published elsewhere [ 52- 55].
Currently, the World Health Organization (WHO) recommends an RT-PCR based on primers published in 1998 (www.who.int/csr/disease/avian_influenza/guidelines/RecAIlabtestsAug07.pdf).
A cDNA encoding a laminin receptor protein (Lamr) has been isolated from hemocytes of the Pacific white shrimp Penaeus (Litopenaeus) vannamei (Pv), based on primers designed from a previously published Lamr sequence of a Taura syndrome virus (TSV) binding protein of the black tiger shrimp Penaeus monodon (Pm).
Amplification primers and SNaPshot primers were based on ones published previously [Paracchini et al., 2002; Bosch et al., 2006], with additional primers based on published sequences [Y Chromosome Consortium, 2002].
PCR primers were based on conserved portions of aligned sequences from the National Center for Biological Information's (NCBI) GenBank database, and designed with the aid of Primer 3 v 0.4.0 [29], or based on previously published primers (Table 3).
Based on previously published primers for cox1 [ 54], cytb [ 55] and rrnL [ 56] initial genome fragments were amplified and provided the basis for specific primer design.
A multiplex reverse transcription PCR (RT-PCR) capable of distinguishing type A from type B influenza virus was developed based on previously published primers (9 ).
We designed PCR primers based on the published sequence and confirmed that the cDNA isolated from EML cells was identical to the published sequence.
An RT transcript from T47D cells was isolated, using RT-PCR with primers based on the published T47D endogenous retroviral pol sequences.
This gene was designed by the computer programme Juniper, which designed six 60 mer overlapping oligonucleotide primers based on the published amino acid sequence of DI [ 6].
PCR using primers based on the published sequences of bovine intestinal [ 4] and non-specific AP [ 3] established that the nasal mucosa PCR product is identical to the bovine non intestinal AP messenger and was not related to intestinal AP.
