Sentence examples for based on primers located from inspiring English sources

Detection of HPMV was based on primers located in the L gene.

Positive amplification and sequencing of L. luteus intergenic regions, based on PCR primers located on M. t runcatula adjacent genes, suggested the existence of microscale synteny between these legume species.

Primer sequences are based on primers published elsewhere [ 52- 55].

The random primed method is based on primer design and the optimization of the PCR protocol.

The first was based on the vectorette PCR and the second employed primers located in conserved portions of exons 1 and 3 or 4 to amplify the intervening fragments from cDNA.

The UP (universal primer) primer was designed based on the primers of suppression PCR [ 25].

Gene-specific PCR primers located within exons or spanning exon junctions were designed based on human gene sequences obtained from Ensembl http://www.ensembl.org/.org/

Based on the amelogenin gene located on the X and Y chromosomes, a pair of primers was designed and the system of PCR was optimized.

The primers (Additional file 1: Table S2) used to amplify the ORFs were designed based on the DNA sequences located in the 5′ and 3′ UTR of each gene.

A multiplex PCR based on these hdc primers and recently designed primers targeting the tyrosine decarboxylase (tyrdc) gene was created.

SSR primers were designed based on seven Mahonia primer pairs.