Sentence examples for based on primers designed from inspiring English sources
A multiplex reverse-transcription polymerase chain assay (mRT-PCR) was developed, based on primers designed to distinguish the A and B types of beet necrotic yellow vein virus (BNYVV).
A RT-PCR technique, based on primers designed complementary to a common conserved region and divergent 3′ sequences of the bean chs family, was developed to detect the expression of individual members of the chs family.
A cDNA encoding a laminin receptor protein (Lamr) has been isolated from hemocytes of the Pacific white shrimp Penaeus (Litopenaeus) vannamei (Pv), based on primers designed from a previously published Lamr sequence of a Taura syndrome virus (TSV) binding protein of the black tiger shrimp Penaeus monodon (Pm).
The universal and differential detection was based on primers designed to amplify a fragment of the 5′ non-coding genome region for the detection of pestiviruses and a fragment of the NS5B gene for the detection of classical swine fever virus.
However, in contrast to what was reported by Poon et al. the sensitivity and specificity of LAMP compared to a nested PCR based on primers designed by Singh et al. [18] were shown to be 79.1% and 58.3% respectively when heat treatment for DNA extraction was used [15].
CRISPR sequences were amplified based on primers designed from the palindromic repeat sequences of various CRISPRs (Additional file 1: Table S7).
Nested PCR assay was established based on primers design and applied to seven fecal samples from road killed birds containing nematode and/or trematode eggs.
Mature trnK product (intron removed) based on primer design, would result in a band slightly larger than 50 base pairs (bp) while precursor RNA still containing intron would result in a band of ~2800 bp.
The failure of these gene targets to reach significance in the qRT-PCR analysis may reflect the variability in microarray preprocessing algorithms as well as differences in expression values based on primer design as primers used in this study were not designed to align with Affymetrix probe locations.
The primers used in the LAMP detection technique are based on six primers designed for the target sequence (Abdul-Ghani et al. 2012; Ahmad and Hashsham 2012; Deborggraeve and Buscher 2012; Ebenezer et al. 2012; Njiru 2012).
