Primer sequences are based on primers published elsewhere [ 52- 55].
Master regulatory proteins were searched based on the designated workflow of the GeneXplain software.
Hospitals were then selected for participation based on two criteria: designated as rural by the US Department of Agriculture and designated as fully medically underserved [ 21].
MSP distinguishes unmethylated from methylated alleles based on the sequence changes created by bisulfite treatment of DNA, which are recognized by PCR with one of two pairs of primers designated for either methylated or unmethylated DNA.
Based on the sequence of the SSU-rDNA gene of E. moshkovskii Laredo (GenBank accession no. AF 149906), a nested set of primers (designated Em-1/Em-2 anEm-1/nEm-2m-2) was designed (unpub. data) and used to detect E. moshkovskii in stool DNA (Table 1).
The new verification method has been introduced based on designated characteristic points.
Based on the sequences of the 16S-like rRNA gene of E. histolytica, E. dispar and E. moshkovskii, nested sets of primers (designated E-1/E-2, EH-1/EH-2, ED-1/ED-2 and Mos-1/Mos-2) were used as described previously [ 21].
Based on the sequences of the small subunit rRNA genes (SSU-rDNA) of E. histolytica and E. dispar, nested sets of primers (designated E-1/E-2, Eh-1/Eh-2, and Ed-1/Ed-2) were used, as described (11 ), to detect E. histolytica and E. dispar in stool specimens (Table 1).
The UP (universal primer) primer was designed based on the primers of suppression PCR [ 25].
Reverse primers (designated WC1groups1,2-rev, WC1group3-rev and WC1group4-rev) were designed to specifically amplify each of three sequence-related subgroups of WC1 transcripts based on the WC1 genes identified here.
Effective number of persons in vehicle i, based on the space offer, designated occupancy rate.
