Sentence examples similar to based on primers derived from inspiring English sources
A PCR screening system based on primer sets derived from genetically mapped ESTs on chromosome 3H was used for clone selection in a BAC library developed from cultivar "Haruna Nijo".
Primer sequences are based on primers published elsewhere [ 52- 55].
The random primed method is based on primer design and the optimization of the PCR protocol.
The primer pairs for β-tub and lys2 were designed based on sequences derived from primers used in previous studies, respectively [ 16, 17].
RNA from 2 ferret HEVs was randomly selected, and the full-length genome was amplified by using reverse transcription PCR with primers based on nucleotide sequences derived from strains from the Netherlands and United States (Table 1).
Specific primers were designed for 3'- and 5'-end amplification of the HCT transcript, based on the derived incomplete cDNA sequence (Table 1).
Gene-specific primers (5′-GAA GGC GGA CAT GTT GGT CGG GCA G-3′ and 5′-TGT GTC GTC CGA ACA GAA CAG CTG A-3′) were designed based on sequence derived from 5′-RACE and Ensembl database (Zv8).
Genome insertion points of Tn2010 were heterogeneous amongst MDR 19A and 19F based on polymerase chain reaction (PCR) using primers derived from whole genome sequencing.
In 25 patients and 15 healthy women positive for TTV DNA in both cervical smears and serum samples, the TTV genotype was determined by a PCR assay based on amplification with TTV group-specific primers derived from the conserved non-coding region [ 18].
The strains were identified on the basis of phenotypic tests and genetic analysis based on polymerase chain reaction amplification using species-specific primers derived from 16S rRNA sequences, as previously reported [ 11].
