Sentence examples for based on primer specificity from inspiring English sources

Consequently, we developed both JP2-clone- and non-JP2-clone-specific quantification methods based on primer specificity and the qPCR technique.

These methodologies are broadly used but are based on primer or probe specificity and can miss some expressions due to a lowest affinity for isoforms of the same mature microRNA.

Up to three primer pairs per gene were selected based on primer parameters as well as predicted gene specificity (Wang and Seed 2003).

Primer sequences are based on primers published elsewhere [ 52- 55].

Primer specificity was confirmed based on each primer pair sequence against soybean genome sequences by BLASTing (http://www.phytozome.net/soybean) using the BLASTN algorithm.

The detection and quantification of known SNPs are primarily based on the specificity of target hybridization and enzyme discriminations, such as specific enzymatic cleavage, and single base primer extension.

Probes were ranked based on their specificity to clusters, specificity to subclusters, and probe-design parameters.

Primers were chosen based on 3' specificity for the CXCR1 gene (Table S2), in order to avoid mis-amplification of the CXCR1 pseudo-gene (gi 186372).

The UP (universal primer) primer was designed based on the primers of suppression PCR [ 25].

The specificity is mostly based on the primer mismatch at the 3′ end to fungal and plant sequences (Table  2).

The primers designed using Primer3 were ranked based on predicted gene specificity and assessed using BLAST, searching Drosophila transcriptome and genome sequences.