Sentence examples for based on primer extension from inspiring English sources

Recent technological developments in PCR-based genotyping based on primer extension with universal PCR primers [ 22] have demonstrated very high (~100-plex) multiplexing levels, although the use of common primers does introduce other issues including the greater potential for cross-contamination.

We focus on the SNP genotyping method based on primer extension and mass-spectrometry (PEA/MS).

This target-triggered polymerase activity is monitored in the second signal transduction step based on primer extension reaction coupled with TaqMan probe.

We recently proposed a method of detection of Pol ι activity in animal cell extracts, based on primer extension opposite the template T with a high concentration of only two nucleotides, dGTP and dATP (incorporation of "G" versus "A" method of Gening, abbreviated as "misGvA").

Transcriptional analysis of this gene has identified three potential promoters based on primer extension analysis (Wachi et al., 2006).

The ability of this assay to genotype any SNP regardless of the base pair involved is an important advantage over assays based on primer extension such as PCR.

The PCR profiles started with an initial denaturation of DNA at 94°C for 5 minutes, followed by 35 amplification cycles of denaturation at 94°C for 1 minute, annealing temperatures varied from 55°C to 58°C for 45 seconds based on the primer, extension at 72°C for 1 minute and final extension at 72°C for 7 minutes.

This has led to the development of mutation screens based on arrayed primer extension technology, which enable the simultaneous detection of multiple mutations from one individual.

Primer sequences are based on primers published elsewhere [ 52- 55].

Taken together, when we introduce the contributions of Dr. Sanger in DNA sequencing, we really have to add the information that based on the primer-extension approach developed by Dr. Wu, and Dr. Sanger further refined the methodology and played an important role in DNA sequencing.

1,536 SNPs were genotyped using Illumina's GoldenGate technology based on allele-specific primer extension followed by highly multiplex PCR using universal primers [9].