Sentence examples for based on primer design from inspiring English sources

The random primed method is based on primer design and the optimization of the PCR protocol.

Mature trnK product (intron removed) based on primer design, would result in a band slightly larger than 50 base pairs (bp) while precursor RNA still containing intron would result in a band of ~2800 bp.

A multiplex reverse-transcription polymerase chain assay (mRT-PCR) was developed, based on primers designed to distinguish the A and B types of beet necrotic yellow vein virus (BNYVV).

A RT-PCR technique, based on primers designed complementary to a common conserved region and divergent 3′ sequences of the bean chs family, was developed to detect the expression of individual members of the chs family.

A cDNA encoding a laminin receptor protein (Lamr) has been isolated from hemocytes of the Pacific white shrimp Penaeus (Litopenaeus) vannamei (Pv), based on primers designed from a previously published Lamr sequence of a Taura syndrome virus (TSV) binding protein of the black tiger shrimp Penaeus monodon (Pm).

However, in contrast to what was reported by Poon et al. the sensitivity and specificity of LAMP compared to a nested PCR based on primers designed by Singh et al. [18] were shown to be 79.1% and 58.3% respectively when heat treatment for DNA extraction was used [15].

CRISPR sequences were amplified based on primers designed from the palindromic repeat sequences of various CRISPRs (Additional file 1: Table S7).

PIPSL locus including the coding sequence (CDS) and 1 Kb flanking regions (mainly untranslated regions, UTR) were PCR amplified based on primers designed by Oligo.

PCR based on primers designed from multiple alignments of the mitochondrial cytochrome b gene have identified avian and mammalian hosts of various species of mosquito (Ngo and Kramer, 2003; Kent and Norris, 2005; Molaei et al., 2006; Kent et al., 2006).

Using targeted PCR based on primers designed from the N. punctiforme genome, we were able to amplify and sequence several genes from the genomic region associated with scytonemin biosynthesis of Chlorogloeopsis, and found that their genomic arrangement was very similar to that of N. punctiforme.

A 626 bp band corresponding to a vitamin C transporter (SVCT2) based on the primer design was detected by RT-PCR analysis in all breast cancer cell lines.