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This apparent variation in required annealing temperature was unanticipated based on primer analysis, but it may depend on physical properties of the particular region of the gene targeted or of the primers.
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Transcriptional analysis of this gene has identified three potential promoters based on primer extension analysis (Wachi et al., 2006).
These predictions are: 225±72 (SE) OTUs at 99% level of gene identity based on primer set 3 individual dataset, and 126±42 (SE) and 132±30 (SE) OTUs at 98% level based on primer sets 1 and 2 individual datasets, respectively (the 4th analysis that used primer set 2 at 99% level OTUs did not produce statistically valid total richness predictions).
Denaturing gradient gel electrophoresis (DGGE) analysis based on primers selective for archaea indicated presence of very few methanogens.
In the F2 segregating population of C101A51 × CO39, PCR analysis based on primers 9-Pro-F/9-Pro-R detected a 111-bp fragment in either homozygous or heterozygous patterns in all 134 resistant plants.
PCR analysis based on primers 9-Pro-F/9-Pro-R detected a Pi9-type genotype and a Pi2/Piz-t-type genotype in IRBL9-W and in IRBLz5-CA/IRBLzt-T (Table 2), respectively.
Primer sequences are based on primers published elsewhere [ 52- 55].
Real-time PCR analysis based on A. tamarense primers allowed the detection of dinoflagellate DNA in Mytilus spp. samples exposed to this alga.
The detection of a single nucleotide polymorphism was based on the analysis of primer extension products generated from the genomic DNA that was previously amplified using a chip-based MALDITOF mass spectrometry platform (Sequenom, Inc., California, USA).
The detection of a single nucleotide polymorphism was based on an analysis of primer extension products generated from previously amplified genomic DNA using a chip-based matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry platform (Sequenom, California, USA).
The detection of a single nucleotide polymorphism was based on an analysis of primer extension products generated from previously amplified genomic DNA, using a chip-based matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry platform (Sequenom, California, USA).
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