Sentence examples for based on primer from inspiring English sources

Recent technological developments in PCR-based genotyping based on primer extension with universal PCR primers [ 22] have demonstrated very high (~100-plex) multiplexing levels, although the use of common primers does introduce other issues including the greater potential for cross-contamination.

We substituted TC001980 and TC003552 for TC001930 and TC003522, respectively, in Table one of South, et al. [ 19] based on primer gene identification (A. Dapper, personal communication).

We focus on the SNP genotyping method based on primer extension and mass-spectrometry (PEA/MS).

This target-triggered polymerase activity is monitored in the second signal transduction step based on primer extension reaction coupled with TaqMan probe.

The random primed method is based on primer design and the optimization of the PCR protocol.

These predictions are: 225±72 (SE) OTUs at 99% level of gene identity based on primer set 3 individual dataset, and 126±42 (SE) and 132±30 (SE) OTUs at 98% level based on primer sets 1 and 2 individual datasets, respectively (the 4th analysis that used primer set 2 at 99% level OTUs did not produce statistically valid total richness predictions).

The assay is based on primer-probe energy transfer, and the most important advantage of this is the relative tolerance towards mutations in the target-probe region.

Denaturing gradient gel electrophoresis (DGGE) analysis based on primers selective for archaea indicated presence of very few methanogens.

Several PCR amplification systems based on primers specific for fungal 18S ribosomal RNA genes were tested to design strategies for the assessment of fungal communities in soil.

Nested PCR assay was established based on primers design and applied to seven fecal samples from road killed birds containing nematode and/or trematode eggs.

A multiplex reverse-transcription polymerase chain assay (mRT-PCR) was developed, based on primers designed to distinguish the A and B types of beet necrotic yellow vein virus (BNYVV).