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Firstly, 14 wrongly annotated translation start sites (TLSs) were corrected based on primary transcriptome data.
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Because UFM is based on primary determinants of coding sequences that are conserved throughout the biosphere, it is suitable for cORF classification of any sequence in eukaryote transcriptomes without prior knowledge.
Data collection was based on primary and secondary sources.
Moreover, as this practice is not documented, it is misleading for the construction of secondary databases such as Bgee based on the primary transcriptome data.
The primers were designed based on the transcriptome sequences of the candidate genes (Siah et al. 2016).
With the widespread use of RNA sequencing (RNAseq), the prediction of operons based on actual transcriptome sequencing rather than computational genomics alone is much needed.
Based on the transcriptome data sequences of eight potential reference genes were obtained.
A PIN of E. sinensis was generated based on the transcriptome sequencing.
A total of 10,754 primer pairs were successfully designed based on the transcriptome sequences.
We considered two paths towards finding novel therapeutics based on whole transcriptome profiling information.
Based on the transcriptome, codon usage bias was analyzed and simple sequence repeats (SSRs) were detected.
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