Since library construction was based on peptides previously selected for binding and selectivity to specific RNAs, the number of target-specific clones increased dramatically following in vitro maturation.
CSH theoretical prediction models : Based on our previously selected theoretical prediction models of cross-hybridization [ 17] (Table 2, Model 1), we further construct the BLAST prediction model based on the total length of the sequence alignment and the admissible maximum mismatches within the sequence, as shown in Table 3.
The library was based on a previously selected thiamine pyrophosphate riboswitch.
Typical Nanobody characteristics (such as small size, single-domain nature, and robust folding) make formatting and the construction of second-generation libraries based on a previously selected Nanobody more straightforward [ 14], which can be exploited to optimize binding characteristics to a given antigen more efficiently.
The doses of antagonists were selected based on previously published studies that show selectivity for the individual receptor subtypes [ 18, 29– 329.
The selected outcome measures have been selected based on previously established reliability and validity or based on recommendations by the Netherlands Heart Foundation guidelines for stroke rehabilitation (table 2)[ 26].
The dilutions and concentrations of antibodies were selected based on previously studies in rodent brain documenting their specificity and selectivity [28].
The nucleotide targets of the FAS coding sequence were selected based on previously published experiments (De Schrijver et al, 2003) and computer-based target analysis using siRNA Target Finder (Ambion Inc., Austin, TX, USA).
The type of steel fibers, selection of optimum and practical fiber dosage, and suitable fiber factors to cast the beams were selected based on previously carried out experiments at the University of Houston (Tadepalli et al. 2013).
Prospective EDIII cross-reactive epitope residues were also selected based on previously described procedural algorithms [20], [44].
