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This is a very restricted approach since TREs can be very degenerated and, based on previously characterized TREs, position one (as well as other positions) could potentially have any of the four nucleotides.
The enrichment analysis based on previously characterized clusters of co-expressed cell cycle genes that share conserved promoter motifs [25], indicated that the RpoD housekeeping sigma factor, the FixK transcription factor, the SigT ECF sigma factor, and the CtrA cell cycle master regulator play significant roles in the response of C. crescentus to carbon starvation.
Based on previously characterized mutants and on our finding that the Ndc80 complex co-purifies with Cik1, this supports a key role for Kar3 at the kinetochore.
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The apical 12 mm of each root was sectioned into three regions based on previously-characterized longitudinal expansion rate profiles: region 1, 0 3 mm plus the root cap; region 2, 3 7 mm; region 3, 7 12 mm.
To learn more about iron transport systems in general and in cyanobacteria particularly we searched for genes coding for TBDTs based on previously experimentally characterized TBDTs.
In this regard, analyses of DUSP-deficient mice frequently show that the phosphorylation of MAP kinases is not accordingly enhanced based on their previously characterized substrate specificity in vitro.
Based on the previously characterized Glu-A3 genes, a pair of specific primers (A3-F and A3-R) for the Glu-A3 locus was designed and used to amplify the Glu-A3a allele from CS.
The aim of this study was to develop a simple and direct assay for HIV-1 release based on our previously characterized fluorescently labelled HIV derivative [ 19, 20], which should allow the quantitation of particles in tissue culture supernatants from virus producing cells by direct measurement of fluorescence intensity.
Essentially, we have carried out a retrospective genotype-phenotype analysis based on previously published studies which characterized the phenotypic traits of patients with deletions encompassing DIA1R.
Both works are based on previously existing material.
ISH experiments are based on previously published protocols for cichlids33.
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