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Based on previous unpublished data, we have hypothesized that a 50-min period would be enough for PaCO2 equilibrium after each change on ECMO settings.
Frames 13 15 min were selected based on previous (unpublished) work which optimised the method by evaluating the performance using a range of possible reference frames for [11C]--PHNO.
Questions were chosen based on previous, unpublished qualitative research performed in the five aforementioned countries.
Twenty fish were injected intramuscularly with A. hydrophila at a dose of 1 × 108 cfu/200 g (This dose was chosen based on previous unpublished data) of fish.
C4, B100 and PON1 were selected for further analysis in a larger independent cohort of patient samples (based on previous unpublished findings), with both C4 and PON1 confirmed to be significantly associated with bone disease.
An additional power calculation assuming 10 COPD patients per practice, and a correlation of SGRQ within primary care practices scores of 0.14 (based on previous unpublished studies in our group), the total number of patients needed to achieve a power of 0.8 is 462.
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The choice of cyclophilin was based on previous studies (unpublished data) in which no differences in the level of expression of this gene were found on PBMCs from pigs experimentally inoculated with ASFV of different virulence.
The choice of the indicated concentrations of Pt in the MTT assay was based on previous research of our group (unpublished data).
A sample size calculation was carried out, based on previous results from a quality control study (unpublished) at the same clinic.
Xanafide was dosed at the maximum tolerated dose 30 mg kg−1, on the basis of reported in vivo studies with amonafide (Dorr et al, 2001), and docetaxel at 5 and 12.5 mg kg−1 based on previous paclitaxel NCI tested doses (Hollingshead M, unpublished data).
This choice of 25% was based on previous inspection of normal controls in prior studies (unpublished), where the hyporeflective choriocapillaris band was discontinuous for up to 25% of the scan length in some eyes.
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